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Canonical Sequence Approximation

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Murine V-H domains

The sequences underlying this analysis have been taken from the Kabat database of sequences of proteins of immunological interest (5th edition). They have been manually edited wherever necessary to align insertions at the respective positions where they have been crystallographically determined. Only the six most frequent residues are given in this table.

ALL MURINE HEAVY CHAIN VARIABLE DOMAINS
Heavy chains subgroup I (A) V region - Mouse
Heavy chains subgroup I (B) V region - Mouse
Heavy chains subgroup II (A) V region - Mouse
Heavy chains subgroup II (B) V region - Mouse
Heavy chains subgroup II (C) V region - Mouse
Heavy chains subgroup III (A) V region - Mouse
Heavy chains subgroup III (B) V region - Mouse
Heavy chains subgroup III (C) V region - Mouse
Heavy chains subgroup III (D) V region - Mouse
Heavy chains subgroup V (A) V region - Mouse
Heavy chains subgroup V (B) V region - Mouse
Heavy chains subgroup misc. V region - Mouse
 
 
  +--- Position
  |
  |   +--- Number of observations (not counting gaps)
  |   |
  |   |      +--- Residue type
  |   |      |
  V   V      V v--- Observed frequency in sample
  1 (  964): E:0.621 Q:0.293 D:0.078 K:0.004 H:0.001 L:0.001
  2 (  964): V:0.971 I:0.024 M:0.002 A:0.001 S:0.001 X:0.001
  3 (  968): Q:0.668 K:0.279 M:0.019 H:0.014 N:0.007 R:0.004
  4 (  988): L:0.986 V:0.010 F:0.002 H:0.001 P:0.001
  5 ( 1007): Q:0.518 V:0.277 K:0.085 L:0.069 E:0.032 D:0.007
  6 ( 1003): E:0.516 Q:0.472 R:0.005 V:0.003 D:0.001 G:0.001
  7 ( 1020): S:0.855 P:0.112 T:0.025 V:0.003 E:0.001 F:0.001
  8 ( 1018): G:0.970 E:0.011 D:0.005 V:0.005 A:0.003 S:0.002
  9 ( 1020): G:0.364 P:0.323 A:0.269 T:0.025 S:0.013 D:0.002
 10 ( 1032): E:0.462 G:0.455 S:0.038 V:0.020 D:0.014 K:0.004
 11 ( 1045): L:0.974 V:0.009 I:0.005 A:0.003 F:0.002 R:0.002
 12 ( 1023): V:0.919 A:0.022 M:0.021 K:0.018 G:0.011 L:0.004
 13 ( 1003): K:0.504 Q:0.310 R:0.117 A:0.049 E:0.006 G:0.004
 14 ( 1065): P:0.890 A:0.054 S:0.032 L:0.014 T:0.009 H:0.001
 15 ( 1065): G:0.829 S:0.157 K:0.005 R:0.003 W:0.003 F:0.002
 16 ( 1053): A:0.331 G:0.312 Q:0.161 S:0.096 R:0.036 T:0.028
 17 ( 1148): S:0.928 T:0.055 P:0.007 L:0.004 A:0.003 Q:0.002
 18 ( 1147): V:0.502 L:0.422 M:0.042 R:0.026 N:0.002 Q:0.002
 19 ( 1139): K:0.663 S:0.172 R:0.133 G:0.009 N:0.007 Q:0.006
 20 ( 1163): L:0.586 I:0.220 M:0.176 V:0.013 A:0.001 H:0.001
 21 ( 1148): S:0.840 T:0.153 A:0.002 L:0.002 P:0.002 I:0.001
 22 ( 1124): C:0.999 K:0.001
 23 ( 1130): K:0.446 A:0.268 T:0.165 S:0.060 V:0.034 E:0.018
 24 ( 1122): A:0.668 V:0.153 T:0.144 G:0.016 S:0.007 F:0.004
 25 ( 1150): S:0.897 T:0.087 A:0.008 F:0.002 G:0.002 P:0.002
 26 ( 1101): G:0.989 D:0.006 R:0.002 E:0.001 L:0.001 Y:0.001
 27 ( 1098): Y:0.522 F:0.427 D:0.033 I:0.006 H:0.004 S:0.003
 28 ( 1150): T:0.657 S:0.190 N:0.050 D:0.040 A:0.034 I:0.009
 29 ( 1162): F:0.787 I:0.115 L:0.086 S:0.005 V:0.003 G:0.001
 30 ( 1156): T:0.616 S:0.306 K:0.049 N:0.009 I:0.005 P:0.005
 31 ( 1155): S:0.454 D:0.290 N:0.081 G:0.052 R:0.049 T:0.042
 32 ( 1163): Y:0.687 F:0.132 T:0.040 G:0.038 D:0.034 S:0.028
 33 ( 1162): Y:0.363 W:0.239 G:0.228 A:0.040 L:0.025 T:0.025
 34 ( 1148): M:0.619 I:0.206 V:0.084 W:0.037 A:0.016 L:0.013
 35 ( 1136): N:0.298 H:0.283 S:0.159 E:0.110 W:0.030 Y:0.030
 35a(   46): -:0.959 N:0.026 S:0.005 H:0.003 V:0.003 I:0.002
 35b(    7): -:0.994 S:0.004 F:0.001 G:0.001
 36 ( 1135): W:0.994 R:0.004 C:0.001 K:0.001 S:0.001
 37 ( 1071): V:0.899 I:0.079 A:0.007 M:0.006 L:0.005 F:0.004
 38 ( 1103): K:0.499 R:0.471 C:0.005 M:0.005 N:0.005 G:0.003
 39 ( 1073): Q:0.932 K:0.046 E:0.008 R:0.005 I:0.003 L:0.002
 40 ( 1082): R:0.375 P:0.172 S:0.159 A:0.095 T:0.085 F:0.072
 41 ( 1060): P:0.875 H:0.093 S:0.014 N:0.007 T:0.007 L:0.002
 42 ( 1041): G:0.793 E:0.172 D:0.010 A:0.009 I:0.008 R:0.005
 43 ( 1075): K:0.540 Q:0.306 N:0.063 R:0.058 H:0.023 A:0.002
 44 ( 1020): G:0.607 R:0.137 S:0.102 K:0.069 A:0.058 T:0.015
 45 ( 1080): L:0.978 P:0.009 F:0.005 Q:0.003 R:0.002 V:0.002
 46 ( 1072): E:0.961 K:0.022 D:0.007 A:0.003 G:0.002 Q:0.002
 47 ( 1041): W:0.935 Y:0.032 L:0.021 H:0.006 F:0.003 C:0.001
 48 ( 1062): I:0.617 L:0.154 V:0.139 M:0.085 S:0.004 W:0.001
 49 ( 1074): G:0.749 A:0.240 V:0.007 T:0.002 D:0.001 S:0.001
 50 ( 1102): Y:0.236 R:0.127 E:0.103 V:0.095 A:0.092 D:0.075
 51 ( 1110): I:0.898 S:0.069 F:0.010 L:0.006 T:0.005 V:0.005
 52 ( 1108): N:0.332 R:0.152 D:0.123 S:0.117 Y:0.112 W:0.094
 52a(  928): P:0.537 -:0.166 N:0.127 S:0.084 T:0.023 L:0.022
 52b(  171): -:0.843 K:0.152 N:0.004 A:0.001 E:0.001
 52c(  172): -:0.842 A:0.108 S:0.028 P:0.007 G:0.005 T:0.005
 53 ( 1093): G:0.298 N:0.245 Y:0.141 A:0.089 S:0.089 D:0.071
 54 ( 1109): N:0.322 G:0.236 S:0.234 D:0.147 K:0.023 T:0.018
 55 ( 1102): G:0.638 Y:0.152 S:0.123 D:0.037 V:0.016 T:0.011
 56 ( 1117): S:0.209 Y:0.197 T:0.170 G:0.137 N:0.081 D:0.065
 57 ( 1120): T:0.821 I:0.111 P:0.021 S:0.014 L:0.008 N:0.008
 58 ( 1125): N:0.248 K:0.203 Y:0.146 E:0.122 S:0.082 H:0.049
 59 ( 1127): Y:0.968 F:0.018 N:0.004 H:0.004 S:0.003 C:0.001
 60 ( 1127): N:0.585 S:0.130 A:0.097 P:0.068 T:0.050 D:0.043
 61 ( 1132): E:0.337 P:0.168 Q:0.140 A:0.131 D:0.123 S:0.072
 62 ( 1134): K:0.480 S:0.319 A:0.087 T:0.046 N:0.022 D:0.018
 63 ( 1133): F:0.554 V:0.225 L:0.191 I:0.016 M:0.011 C:0.002
 64 ( 1119): K:0.824 Q:0.059 M:0.053 I:0.024 R:0.013 E:0.011
 65 ( 1086): G:0.587 S:0.252 D:0.110 N:0.018 R:0.012 T:0.010
 66 ( 1123): K:0.541 R:0.451 Q:0.004 P:0.003 E:0.002
 67 ( 1143): A:0.393 F:0.312 T:0.128 L:0.098 I:0.057 V:0.006
 68 ( 1146): T:0.702 S:0.153 I:0.108 A:0.018 K:0.006 F:0.003
 69 ( 1160): L:0.453 I:0.437 V:0.066 F:0.036 M:0.008
 70 ( 1162): T:0.590 S:0.386 F:0.013 N:0.004 A:0.003 L:0.002
 71 ( 1162): R:0.361 V:0.356 A:0.142 K:0.096 L:0.020 S:0.017
 72 ( 1146): D:0.963 E:0.024 N:0.006 V:0.004 G:0.001 H:0.001
 73 ( 1158): K:0.376 T:0.284 N:0.282 D:0.041 R:0.006 E:0.003
 74 ( 1173): S:0.800 A:0.107 P:0.061 D:0.015 T:0.006 G:0.003
 75 ( 1171): S:0.518 K:0.307 Q:0.116 A:0.024 R:0.020 E:0.004
 76 ( 1109): S:0.660 N:0.296 T:0.012 R:0.007 D:0.006 K:0.006
 77 ( 1107): T:0.686 I:0.126 Q:0.121 S:0.035 N:0.014 M:0.005
 78 ( 1150): A:0.508 L:0.266 V:0.150 Y:0.032 F:0.030 T:0.004
 79 ( 1166): Y:0.812 F:0.156 H:0.021 S:0.003 D:0.002 I:0.002
 80 ( 1172): L:0.515 M:0.454 F:0.020 I:0.005 V:0.004 S:0.001
 81 ( 1164): Q:0.772 K:0.106 E:0.082 G:0.011 D:0.009 H:0.008
 82 ( 1164): L:0.574 M:0.381 I:0.033 V:0.006 F:0.004 S:0.002
 82a( 1136): S:0.428 N:0.361 R:0.151 T:0.022 L:0.011 H:0.008
 82b( 1175): S:0.759 A:0.081 N:0.059 K:0.037 T:0.030 R:0.015
 82c( 1179): L:0.871 V:0.126 P:0.002 -:0.001
 83 ( 1155): T:0.572 R:0.249 Q:0.089 K:0.062 A:0.014 D:0.006
 84 ( 1191): S:0.673 A:0.148 T:0.134 N:0.022 P:0.006 F:0.005
 85 ( 1175): E:0.873 D:0.102 N:0.010 A:0.006 G:0.003 S:0.002
 86 ( 1079): D:0.985 E:0.005 A:0.004 N:0.003 G:0.002 S:0.001
 87 ( 1182): S:0.512 T:0.461 M:0.010 A:0.007 P:0.003 F:0.003
 88 ( 1294): A:0.954 G:0.033 V:0.005 P:0.003 L:0.002 T:0.002
 89 ( 1266): V:0.402 T:0.283 I:0.125 M:0.114 L:0.057 R:0.009
 90 ( 1294): Y:0.989 F:0.004 H:0.002 L:0.002 G:0.001 K:0.001
 91 ( 1357): Y:0.792 F:0.199 H:0.004 N:0.001 S:0.001 C:0.001
 92 ( 1363): C:0.995 S:0.002 P:0.001 V:0.001 Y:0.001 -:0.001
 93 ( 1350): A:0.848 T:0.077 V:0.021 G:0.017 S:0.014 M:0.011
 94 ( 1343): R:0.848 S:0.031 G:0.021 K:0.021 T:0.019 I:0.011
 95 ( 1230): D:0.212 S:0.141 G:0.138 Y:0.112 R:0.056 E:0.055
 96 ( 1135): Y:0.215 G:0.179 R:0.076 D:0.065 S:0.058 N:0.053
 97 ( 1111): Y:0.356 G:0.171 -:0.066 L:0.057 D:0.056 N:0.050
 98 (  983): Y:0.301 G:0.182 -:0.161 D:0.053 S:0.044 L:0.039
 99 (  867): -:0.256 G:0.205 Y:0.147 S:0.117 R:0.050 D:0.038
100 (  684): -:0.405 S:0.133 G:0.130 Y:0.088 D:0.038 W:0.036
100a(  511): -:0.549 S:0.129 Y:0.110 G:0.054 R:0.025 D:0.023
100b(  352): -:0.687 Y:0.120 W:0.044 S:0.033 G:0.022 R:0.020
100c(  173): -:0.844 Y:0.043 S:0.023 G:0.021 W:0.013 D:0.010
100d(   89): -:0.919 Y:0.024 G:0.011 E:0.007 D:0.005 V:0.005
100e(   46): -:0.958 Y:0.017 G:0.009 P:0.005 D:0.004 A:0.002
100f(   26): -:0.976 Y:0.008 D:0.003 F:0.003 P:0.003 G:0.002
100g(   14): -:0.987 Y:0.005 V:0.003 A:0.001 D:0.001 F:0.001
100h(    4): -:0.996 A:0.001 G:0.001 T:0.001 Y:0.001
100i(    1): -:0.999 Y:0.001
100j(  783): Y:0.346 -:0.286 A:0.173 W:0.071 S:0.023 F:0.015
100k(  965): F:0.596 M:0.184 -:0.132 L:0.027 V:0.015 Y:0.012
101 ( 1076): D:0.713 A:0.184 -:0.038 V:0.013 G:0.008 P:0.007
102 ( 1117): Y:0.774 V:0.158 F:0.020 S:0.012 H:0.012 D:0.010
103 ( 1079): W:0.984 A:0.006 L:0.005 D:0.002 V:0.002 G:0.001
104 ( 1032): G:0.984 Y:0.007 A:0.004 H:0.002 S:0.002 T:0.001
105 (  817): Q:0.781 A:0.119 T:0.076 W:0.012 H:0.007 P:0.002
106 (  896): G:0.996 R:0.002 P:0.001 T:0.001
107 (  843): T:0.972 Q:0.012 A:0.007 S:0.005 I:0.002 F:0.001
108 (  823): T:0.478 L:0.267 S:0.233 G:0.012 P:0.004 A:0.001
109 (  792): V:0.681 L:0.306 T:0.014
110 (  794): T:0.976 L:0.010 A:0.008 I:0.001 N:0.001 P:0.001
111 (  749): V:0.988 A:0.004 L:0.003 C:0.001 F:0.001 G:0.001
112 (  727): S:0.982 T:0.017 G:0.001
113 (  676): S:0.722 A:0.257 V:0.015 E:0.003 P:0.001 T:0.001

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