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Canonical Sequence Approximation

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Human V-H domains

The sequences underlying this analysis have been taken from the Kabat database of sequences of proteins of immunological interest (5th edition). They have been manually edited wherever necessary to align insertions at the respective positions where they have been crystallographically determined. Only the six most frequent residues are given in this table.

HUMAN HEAVY CHAIN VARIABLE DOMAINS:
Heavy chains subgroup I V region - Human
Heavy chains subgroup II V region - Human
Heavy chains subgroup III V region - Human
 
 
  +--- Position
  |
  |   +--- Number of observations (not counting gaps)
  |   |
  |   |      +--- Residue type
  |   |      |
  V   V      V v--- Observed frequency in sample
  1 (  291): E:0.616 Q:0.346 D:0.014 G:0.014 A:0.003 L:0.003
  2 (  293): V:0.887 M:0.027 L:0.024 S:0.020 I:0.017 A:0.007
  3 (  291): Q:0.852 H:0.034 R:0.027 T:0.027 E:0.014 V:0.014
  4 (  282): L:0.975 V:0.011 A:0.007 D:0.004 M:0.004
  5 (  276): V:0.645 Q:0.148 L:0.120 R:0.022 M:0.014 N:0.014
  6 (  267): E:0.693 Q:0.263 A:0.022 D:0.011 G:0.007 R:0.004
  7 (  265): S:0.951 W:0.019 X:0.015 T:0.008 A:0.004 N:0.004
  8 (  266): G:0.989 S:0.008 T:0.004
  9 (  274): G:0.624 A:0.193 P:0.164 S:0.011 E:0.004 H:0.004
 10 (  271): G:0.638 E:0.192 D:0.081 A:0.070 T:0.011 V:0.007
 11 (  270): L:0.681 V:0.270 F:0.030 S:0.019
 12 (  267): V:0.757 K:0.154 I:0.026 N:0.022 L:0.015 A:0.007
 13 (  247): K:0.474 Q:0.428 R:0.049 E:0.034 G:0.004 H:0.004
 14 (  251): P:0.968 A:0.012 K:0.008 G:0.004 L:0.004 S:0.004
 15 (  244): G:0.783 S:0.156 T:0.033 P:0.016 K:0.008 E:0.004
 16 (  243): G:0.488 E:0.131 Q:0.107 A:0.094 R:0.082 S:0.066
 17 (  234): S:0.766 T:0.204 A:0.009 F:0.009 P:0.004 R:0.004
 18 (  244): L:0.812 V:0.155 M:0.008 A:0.004 E:0.004 F:0.004
 19 (  242): R:0.545 K:0.240 S:0.161 T:0.037 A:0.012 Q:0.004
 20 (  246): L:0.736 V:0.191 I:0.061 E:0.004 R:0.004 X:0.004
 21 (  218): S:0.729 T:0.234 G:0.009 I:0.009 A:0.005 D:0.005
 22 (  217): C:0.991 R:0.005 S:0.005
 23 (  231): A:0.558 K:0.203 T:0.117 E:0.048 V:0.022 I:0.013
 24 (  235): A:0.638 V:0.174 G:0.064 I:0.055 T:0.030 F:0.026
 25 (  226): S:0.951 Y:0.027 F:0.009 C:0.004 K:0.004 T:0.004
 26 (  225): G:0.956 E:0.013 A:0.009 D:0.009 S:0.009 V:0.004
 27 (  213): F:0.559 Y:0.164 G:0.150 D:0.080 S:0.019 L:0.014
 28 (  203): T:0.571 S:0.286 I:0.049 N:0.049 P:0.015 A:0.005
 29 (  207): F:0.749 V:0.111 I:0.068 L:0.053 T:0.010 A:0.005
 30 (  202): S:0.762 T:0.119 N:0.035 G:0.020 R:0.020 A:0.010
 31 (  199): S:0.482 T:0.136 D:0.104 N:0.087 G:0.060 K:0.040
 32 (  202): Y:0.535 S:0.144 N:0.083 A:0.069 D:0.031 G:0.030
 33 (  197): A:0.269 Y:0.162 G:0.147 W:0.117 S:0.091 T:0.066
 34 (  200): M:0.520 I:0.210 W:0.070 A:0.055 Y:0.050 V:0.040
 35 (  196): S:0.372 H:0.235 N:0.077 A:0.061 G:0.051 Y:0.046
 35a(   33): -:0.824 W:0.096 V:0.043 G:0.016 S:0.016 N:0.005
 35b(   27): -:0.856 N:0.064 G:0.037 S:0.032 A:0.005 R:0.005
 36 (  192): W:0.990 M:0.005 T:0.005
 37 (  193): V:0.741 I:0.228 L:0.021 G:0.005 Q:0.005
 38 (  190): R:0.989 P:0.005 V:0.005
 39 (  190): Q:0.979 T:0.011 G:0.005 R:0.005
 40 (  191): A:0.634 P:0.199 S:0.073 M:0.052 G:0.010 V:0.010
 41 (  187): P:0.914 S:0.043 T:0.021 A:0.005 L:0.005 Q:0.005
 42 (  187): G:0.925 S:0.064 P:0.005 R:0.005
 43 (  186): K:0.683 Q:0.183 R:0.124 E:0.005 H:0.005
 44 (  186): G:0.882 A:0.048 S:0.043 R:0.027
 45 (  186): L:0.978 P:0.022
 46 (  185): E:0.956 Q:0.039 V:0.005
 47 (  184): W:0.989 S:0.011
 48 (  185): V:0.481 M:0.222 I:0.173 L:0.124
 49 (  185): G:0.600 S:0.216 A:0.162 E:0.005 L:0.005 T:0.005
 50 (  185): R:0.146 W:0.146 V:0.119 A:0.114 G:0.081 Y:0.081
 51 (  185): I:0.822 T:0.081 R:0.027 V:0.022 K:0.016 M:0.011
 52 (  184): S:0.250 Y:0.239 N:0.123 K:0.060 I:0.054 D:0.050
 52a(  141): -:0.230 P:0.180 Y:0.153 G:0.126 N:0.066 V:0.055
 52b(   34): -:0.814 K:0.115 R:0.060 G:0.005 Y:0.005
 52c(   22): -:0.880 T:0.044 V:0.033 S:0.022 A:0.011 G:0.005
 53 (  184): S:0.228 D:0.163 Y:0.125 G:0.109 N:0.082 H:0.054
 54 (  183): G:0.328 S:0.202 D:0.129 N:0.112 K:0.082 F:0.055
 55 (  182): G:0.544 S:0.181 D:0.085 W:0.066 Y:0.060 N:0.020
 56 (  182): S:0.231 D:0.182 N:0.147 T:0.143 Y:0.077 G:0.060
 57 (  184): T:0.582 K:0.120 N:0.065 A:0.054 I:0.054 P:0.022
 58 (  183): Y:0.322 N:0.216 D:0.139 R:0.060 H:0.055 T:0.038
 59 (  184): Y:0.908 F:0.043 N:0.016 S:0.011 D:0.005 G:0.005
 60 (  183): A:0.579 N:0.153 S:0.104 T:0.055 R:0.044 G:0.027
 61 (  184): D:0.277 P:0.239 Q:0.174 A:0.141 V:0.076 T:0.033
 62 (  185): S:0.686 K:0.146 P:0.065 N:0.038 G:0.016 R:0.016
 63 (  186): V:0.511 L:0.247 F:0.215 S:0.011 A:0.005 K:0.005
 64 (  186): K:0.581 Q:0.274 R:0.054 N:0.032 E:0.022 T:0.022
 65 (  186): G:0.688 S:0.237 T:0.032 A:0.016 D:0.011 E:0.011
 66 (  186): R:0.935 Q:0.054 H:0.005 I:0.005
 67 (  186): F:0.462 V:0.409 I:0.065 L:0.054 A:0.005 S:0.005
 68 (  186): T:0.914 I:0.038 A:0.016 S:0.011 K:0.005 N:0.005
 69 (  187): I:0.791 M:0.139 V:0.032 D:0.005 F:0.005 G:0.005
 70 (  187): S:0.684 T:0.214 N:0.070 L:0.032
 71 (  187): R:0.529 V:0.160 A:0.107 P:0.064 T:0.053 K:0.043
 72 (  186): D:0.902 N:0.071 K:0.016 E:0.011
 73 (  185): T:0.368 N:0.266 D:0.177 K:0.070 E:0.059 A:0.011
 74 (  186): S:0.946 A:0.048 L:0.005
 75 (  187): K:0.674 T:0.139 I:0.070 R:0.027 A:0.021 F:0.021
 76 (  187): N:0.701 S:0.251 K:0.027 R:0.011 T:0.005 Y:0.005
 77 (  187): T:0.615 Q:0.273 S:0.048 M:0.021 L:0.016 P:0.011
 78 (  186): L:0.364 A:0.273 F:0.235 V:0.096 I:0.005 M:0.005
 79 (  187): Y:0.638 S:0.239 F:0.059 V:0.048 H:0.005 M:0.005
 80 (  187): L:0.782 M:0.207 N:0.005 -:0.005
 81 (  187): Q:0.529 E:0.205 K:0.122 R:0.032 T:0.032 N:0.027
 82 (  194): M:0.497 L:0.421 W:0.051 V:0.015 I:0.010 -:0.005
 82a(  195): N:0.442 S:0.291 R:0.077 T:0.066 D:0.053 G:0.020
 82b(  194): S:0.795 N:0.082 R:0.051 G:0.026 T:0.021 A:0.010
 82c(  197): L:0.701 V:0.234 M:0.041 G:0.010 A:0.005 D:0.005
 83 (  197): R:0.528 T:0.239 K:0.122 D:0.041 E:0.020 Q:0.015
 84 (  198): A:0.495 P:0.182 S:0.177 T:0.051 I:0.035 V:0.030
 85 (  198): E:0.591 A:0.172 D:0.126 S:0.051 V:0.045 G:0.015
 86 (  198): D:0.975 T:0.010 V:0.010 N:0.005
 87 (  198): T:0.929 S:0.035 G:0.010 M:0.010 A:0.005 Q:0.005
 88 (  198): A:0.939 G:0.040 P:0.005 T:0.005 V:0.005 Y:0.005
 89 (  198): V:0.768 L:0.066 M:0.056 T:0.045 I:0.040 F:0.010
 90 (  199): Y:0.980 F:0.010 A:0.005 I:0.005
 91 (  199): Y:0.930 F:0.045 C:0.015 R:0.005 T:0.005
 92 (  198): C:0.990 A:0.005 M:0.005
 93 (  198): A:0.838 T:0.076 V:0.061 H:0.005 K:0.005 N:0.005
 94 (  198): R:0.596 K:0.162 T:0.051 G:0.045 P:0.045 Q:0.025
 95 (  161): G:0.174 D:0.120 E:0.099 A:0.093 N:0.092 P:0.068
 96 (  159): P:0.168 R:0.130 G:0.112 L:0.062 V:0.062 Y:0.062
 97 (  156): G:0.170 P:0.094 V:0.094 E:0.088 T:0.069 S:0.063
 98 (  155): G:0.152 Y:0.101 L:0.095 D:0.087 V:0.076 S:0.063
 99 (  143): G:0.172 Y:0.108 T:0.102 -:0.089 A:0.076 E:0.070
100 (  131): -:0.171 S:0.165 Y:0.146 G:0.095 V:0.070 R:0.051
100a(  110): -:0.304 G:0.146 S:0.095 D:0.046 A:0.044 L:0.044
100b(   99): -:0.369 G:0.134 S:0.127 T:0.076 Y:0.045 V:0.038
100c(   92): -:0.410 G:0.122 Y:0.103 D:0.058 S:0.058 P:0.045
100d(   72): -:0.538 Y:0.058 G:0.051 S:0.051 C:0.045 L:0.038
100e(   62): -:0.600 Y:0.155 S:0.045 F:0.032 G:0.032 A:0.026
100f(   53): -:0.658 Y:0.097 H:0.039 R:0.039 P:0.026 S:0.026
100g(   41): -:0.735 Y:0.084 G:0.065 Q:0.026 S:0.019 D:0.013
100h(   30): -:0.806 Y:0.058 D:0.032 A:0.019 G:0.019 S:0.019
100i(   24): -:0.844 Y:0.039 G:0.026 X:0.019 L:0.013 N:0.013
100j(   80): -:0.481 Y:0.149 A:0.117 W:0.084 F:0.045 G:0.039
100k(  138): F:0.503 M:0.144 L:0.137 -:0.098 D:0.039 V:0.033
101 (  149): D:0.754 A:0.073 R:0.066 N:0.020 Q:0.020 P:0.013
102 (  151): Y:0.368 V:0.224 I:0.112 S:0.086 P:0.072 H:0.053
103 (  154): W:0.955 E:0.013 F:0.013 D:0.006 R:0.006 Y:0.006
104 (  154): G:0.974 Y:0.013 D:0.006 T:0.006
105 (  154): Q:0.798 R:0.104 K:0.045 E:0.013 N:0.013 S:0.013
106 (  155): G:0.987 Y:0.006 -:0.006
107 (  152): T:0.908 S:0.026 V:0.020 G:0.013 I:0.007 L:0.007
108 (  152): L:0.645 T:0.178 M:0.105 P:0.020 K:0.013 R:0.013
109 (  151): V:0.967 L:0.013 I:0.007 M:0.007 X:0.007
110 (  151): T:0.940 S:0.026 I:0.013 A:0.007 H:0.007 V:0.007
111 (  137): V:0.978 I:0.015 T:0.007
112 (  138): S:0.971 T:0.014 R:0.007 V:0.007
113 (  131): S:0.962 P:0.015 A:0.008 L:0.008 T:0.008

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