Difference between revisions of "RPR-Scripting data downloads"

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Scripting Data Downloads
 
Scripting Data Downloads
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(Techniques for accessing databases and downloading data)
 
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<b>Abstract:</b><br />
 
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<li><b>Time management</b>: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.</li>
 
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*<b>Journal</b>: Document your progress in your [[FND-Journal|Course Journal]]. Some tasks may ask you to include specific items in your journal. Don't overlook these.
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*<b>The Central Dogma</b>: Regulation of transcription and translation; protein biosynthesis and degradation; quality control.
 
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This unit builds on material covered in the following prerequisite units:
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Revision as of 12:40, 16 September 2020

Scripting Data Downloads

(Techniques for accessing databases and downloading data)


 


Abstract:

Often we find ourselves in need to automate access to databases that provide their data via Web interfaces, or are only designed to be viewed in Web browsers. This unit discussess three strategies: working with text data that is accessed through GET and POST commands, and parsing simple XML formatted data.

Objectives:
This unit will ...

  • ... introduce the GET and POST verbs to interface with Web servers;
  • ... demonstrate parsing of text and XML responses.

Outcomes:
After working through this unit you ...

  • ... can construct GET and POST queries to Web servers;
  • ... can parse text data and XML data;
  • ... have integrated sample code for this into a utility function.

Deliverables:

  • Time management: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.
  • Journal: Document your progress in your Course Journal. Some tasks may ask you to include specific items in your journal. Don't overlook these.
  • Insights: If you find something particularly noteworthy about this unit, make a note in your insights! page.

    • Prerequisites:
    You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources:

    • The Central Dogma: Regulation of transcription and translation; protein biosynthesis and degradation; quality control.

    This unit builds on material covered in the following prerequisite units:


     


    This page is tagged for revision; expect changes and proceed with caution.


     



     


    Contents

    Many databases provide download links of their holdings, and/or covenient subsets of data, but sometimes we need to access the data piece by piece - either because no bulk download is available, or because the full dataset is unreasonably large. For the odd protein here or there, we may be able to get the information from a Web-page by hand, but this is tedious, and it is easy to make mistakes. Much better to learn how to script data downloads.

    In this unit we will cover three download strategies. Our first example is the UniProt interface from which we will retrieve the FASTA sequence of a protein with a simple GET request for a text file. The second example is to retieve motif annotations from PROSITE - a POST request, with subsequent parsing of a table. The final example is to retrieve XML data from the NCBI via their E-utils interface.


     

    UniProt GET

    Task:

     
    • Open RStudio and load the ABC-units R project. If you have loaded it before, choose File → Recent projects → ABC-Units. If you have not loaded it before, follow the instructions in the RPR-Introduction unit.
    • Choose Tools → Version Control → Pull Branches to fetch the most recent version of the project from its GitHub repository with all changes and bug fixes included.
    • Type init() if requested.
    • Open the file RPR-UniProt_GET.R and follow the instructions.


     

    Note: take care that you understand all of the code in the script. Evaluation in this course is cumulative and you may be asked to explain any part of code.


     


     

    ScanPprosite POST

    ScanProsite is a tool to search for the occurrence of expert-curated motifs in the PROSITE database in a sequence of interest.

    Task:
    ScanProsite uses UniProt IDs. The UniProt ID for yeast Mbp1 is P39678.

    • Navigate to ScanProsite, paste P39678e text field, select Table output for STEP 3, and START THE SCAN.

    You should see four feature hits: the APSES domain, and three ankyrin domain sequences that partially overlap. We could copy and paste the start and end numbers and IDs but that would be lame. Let's get them directly from Prosite instead, because later we will want to fetch a few of these annotations. Prosite does not have a nice API interface like UniProt, but the principles of using R's httr package to send POST requests and retrieve the results are the same. The parameters for the POST request are hidden in the form that your browser sends to the PROSITE Web server. In order to construct our request correctly, we need to get the correct parameter names out from the page. The first step to capture the data from this page via screenscraping is to look into the HTML code of the page.

    (I am writing this section from the perspective of the Chrome browser - I don't think other browsers have all of the functionality that I am describing here. You may need to install Chrome to try this...)

    • Use the menu and access View → Developer → View Source. Scroll through the page. You should easily be able to identify the data table. That's fair enough: each of the lines contain the UniProt ID and we should be able to identify them. But how to send the request to get this page in the first place?
    • Use the browser's back button to go back to the original query form, and again: View → Developer → View Source. This is the page that accepts user input in a so called form via several different types of elements: "radio-buttons", a "text-box", "check-boxes", a "drop down menu" and a "submit" button. We need to figure out what each of the values are so that we can construct a valid POST request. If we get them wrong, in the wrong order, or have parts missing, it is likely that the server will simply ignore our request. These elements are much harder to identify then the lines of feature information, and it's really easy to get them wrong, miss something and get no output. But Chrome has a great tool to help us: it allows you to see the exact, assembled POST header that it sent to the Prosite server!
    • Close the HTML source, and on the scanProsite page with the entry form, open View → Developer → Developer Tools in the Chrome menu. Then click again on START THE SCAN. The Developer Tools page will show you information about what just happened in the transaction that the browser negotiated to retrieve the results page. Click on the Network tab, on All, and then on the last element: PSScan.cgi. This contains the form data. Then click on the Headers tab and scroll down until you see the Form Data. This has all the the required POST elements nicely spelled out. What you are looking for are key value pairs like:
    meta: opt1
    meta1_protein: opt1
    seq: P39678
    etc ...

    These are the field keys, and the required values. You have now reverse-engineered a Web form. Armed with this knowledge we can script it: what worked from the browser should work the same way from an R script.


     

    Task:

     
    • Open RStudio and load the ABC-units R project. If you have loaded it before, choose File → Recent projects → ABC-Units. If you have not loaded it before, follow the instructions in the RPR-Introduction unit.
    • Choose Tools → Version Control → Pull Branches to fetch the most recent version of the project from its GitHub repository with all changes and bug fixes included.
    • Type init() if requested.
    • Open the file RPR-PROSITE_POST.R and follow the instructions.


     

    Note: take care that you understand all of the code in the script. Evaluation in this course is cumulative and you may be asked to explain any part of code.


     


     

    NCBI Entrez E-Utils

    It is has become unreasonably difficult to screenscrape the NCBI site since the actual page contents are dynamically loaded via AJAX. This may be intentional, or just overengineering. While NCBI offers a subset of their data via the eutils API and that works well enough, some of the data that is available to the Web browser's eyes is not served to a program.

    The eutils API returns data in XML format. Have a look at the following URL in your browser to see what that looks like: 

     http://eutils.ncbi.nlm.nih.gov/entrez/eutils/esearch.fcgi?db=protein&term=NP_010227

    An API to NCBIs Entrez system is provided through eUtils.

    Task:
    Browse through the E-utilities Quick Start chapter of the NCBI's Entrez Programming Utilites Handbook for a quick overview.


     

    Task:

     
    • Open RStudio and load the ABC-units R project. If you have loaded it before, choose File → Recent projects → ABC-Units. If you have not loaded it before, follow the instructions in the RPR-Introduction unit.
    • Choose Tools → Version Control → Pull Branches to fetch the most recent version of the project from its GitHub repository with all changes and bug fixes included.
    • Type init() if requested.
    • Open the file RPR-eUtils_XML.R and follow the instructions.


     

    Note: take care that you understand all of the code in the script. Evaluation in this course is cumulative and you may be asked to explain any part of code.


     



    Self-evaluation

    Notes

    Further reading, links and resources

     



     

    If in doubt, ask! If anything about this learning unit is not clear to you, do not proceed blindly but ask for clarification. Post your question on the course mailing list: others are likely to have similar problems. Or send an email to your instructor.


     

    About ...
     
    Author:

    Boris Steipe <boris.steipe@utoronto.ca>

    Created:

    2017-08-05

    Modified:

    2018-12-01

    Version:

    1.0.1

    Version history:

    • 1.0.1 Updtae for Chromes' developer tools layout change
    • 1.0 Working version
    • 0.1 First stub

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