BIN-PDB
The RCSB-PDB Structure Database
(The RCSB PDB database and services)
Abstract:
This unit provides a short introduction to the PDB.
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Objectives:
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Outcomes:
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Deliverables:
Prerequisites:
This unit builds on material covered in the following prerequisite units:
Evaluation
Evaluation: NA
Contents
Task:
- Read the introductory notes on the Protein DataBase (PDB) - the global data repository for protein 3D structure information.
- Read
| Burley et al. (2019) RCSB Protein Data Bank: biological macromolecular structures enabling research and education in fundamental biology, biomedicine, biotechnology and energy. Nucleic Acids Res 47:D464-D474. (pmid: 30357411) |
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[ PubMed ] [ DOI ] The Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB PDB, rcsb.org), the US data center for the global PDB archive, serves thousands of Data Depositors in the Americas and Oceania and makes 3D macromolecular structure data available at no charge and without usage restrictions to more than 1 million rcsb.org Users worldwide and 600 000 pdb101.rcsb.org education-focused Users around the globe. PDB Data Depositors include structural biologists using macromolecular crystallography, nuclear magnetic resonance spectroscopy and 3D electron microscopy. PDB Data Consumers include researchers, educators and students studying Fundamental Biology, Biomedicine, Biotechnology and Energy. Recent reorganization of RCSB PDB activities into four integrated, interdependent services is described in detail, together with tools and resources added over the past 2 years to RCSB PDB web portals in support of a 'Structural View of Biology.' |
The search options in the PDB structure database are as sophisticated as those at the NCBI. For now, we will try a simple keyword search to get us started.
Task:
- Visit the RCSB PDB website at http://www.rcsb.org/pdb
- Briefly orient yourself regarding the database contents and its information offerings and services.
- Enter
Mbp1into the search field. - In your journal, note down the PDB IDs for the three Saccharomyces cerevisiae Mbp1 transcription factor structures your search has retrieved.
- Click on 1BM8 entry and explore the information and services linked from that page.
- On the Structure Summary tab, note that this structure has 1.71Å resolution - which is very high. The protein feature view shows where the domain is located in the full-length protein. This page has a download link, from which you can get the PDB coordinates file, as well as the biological assembly (whenver that is relevant).
- On the 3D View tab, explore the visualization options. The default view is the Mol* molecular viewer. Have a look at the Help page and explore the options. Can you ...
- ...change the color of the molecule?
- ...display a molecular surface?
- ...change the representation to ball-and-stick?
- On the Annotations tab, explore the cross-references. Pursue the link to the "Mlu1-box Binding Protein; DNA-binding Domain" annotation of the CATH database domain annotations at the Topology level, to see how many proteins with a similar fold are held in the PDB.
- Explore the other tabs, and take special note of the Structure Similarity tab. This is one of the important resources, the ability to find and superimpose similar structures helps tremendously in interpreting e.g. conservation patterns in a protein.
Further reading, links and resources
Notes
About ...
Author:
- Boris Steipe <boris.steipe@utoronto.ca>
Created:
- 2017-08-05
Modified:
- 2020-09-22
Version:
- 1.1
Version history:
- 1.1 Maintenance
- 1.0.1 Removed inaccessible article by Costanzo et.al and updated NAR article
- 1.0 First live version
- 0.1 First stub
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