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Keywords: Sequencing technologies; highly parallel; single-molecule and single-cell | |||||||||||
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Deliverables: Time management: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit. Journal: Document your progress in your Course Journal. Some tasks may ask you to include specific items in your journal. Don’t overlook these. Insights: If you find something particularly noteworthy about this unit, make a note in your insights! page. |
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Evaluation: NA: This unit is not evaluated for course marks. |
A basic introduction to “Next Generation Sequencing” concepts and technologies.
Task…
Kashima,
Yukie et al.. (2020). “Single-cell sequencing techniques
from individual to multiomics analyses”. Experimental &
Molecular Medicine 52(9):1419–1427 .
[PMID: 32929221]
[DOI: 10.1038/s12276-020-00499-2]
Kempfer,
Rieke and Ana Pombo. (2020). “Methods for mapping 3D chromosome
architecture”. Nature Reviews. Genetics
21(4):207–226 .
[PMID: 31848476]
[DOI: 10.1038/s41576-019-0195-2]
Ho, Steve
S, Alexander E Urban, and Ryan E Mills. (2020). “Structural
variation in the sequencing era”. Nature Reviews. Genetics
21(3):171–189 .
[PMID: 31729472]
[DOI: 10.1038/s41576-019-0180-9]
Stark,
Rory, Marta Grzelak, and James Hadfield. (2019). “RNA sequencing:
the teenage years”. Nature Reviews. Genetics
20(11):631–656 .
[PMID:
31341269]
[DOI: 10.1038/s41576-019-0150-2]
Sedlazeck, Fritz
J et al.. (2018). “Piercing the dark matter:
bioinformatics of long-range sequencing and mapping”. Nature
Reviews. Genetics 19(6):329–346 .
[PMID: 29599501]
[DOI: 10.1038/s41576-018-0003-4]
Langmead,
Ben and Abhinav Nellore. (2018). “Cloud computing for genomic
data analysis and collaboration”. Nature Reviews. Genetics
19(4):208–219 .
[PMID: 29379135]
[DOI: 10.1038/nrg.2017.113]
A very informative and influential blog about the absolutely newest in the field is written by Lior Pachter, Berkley - “Bits of DNA”. Check it out, and from time to time (continuous).
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