Expected Preparations:

  The Central Dogma:
Regulation of transcription and translation; Protein biosynthesis and degradation; Quality control.
  
  If you are not already familiar with the prior knowledge listed above, you need to prepare yourself from other information sources.  
Keywords: Analysing expression measurements; Microarray: spots to levels; NGS: Reads to counts
Objectives:

This unit will …

  • … introduce microarray and RNAseq methods to estimate mRNA expression levels in the cell.

 Outcomes:

After working through this unit you …

  • … are familar with key methods and terminology in the field.

Deliverables:

Time management: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.

Journal: Document your progress in your Course Journal. Some tasks may ask you to include specific items in your journal. Don’t overlook these.

Insights: If you find something particularly noteworthy about this unit, make a note in your insights! page.

Evaluation:

NA: This unit is not evaluated for course marks.

Contents

This unit introduces microarray and RNAseq methods to estimate mRNA expression levels in the cell.

Task…

Questions, comments

If in doubt, ask! If anything about this contents is not clear to you, do not proceed but ask for clarification. If you have ideas about how to make this material better, let’s hear them. We are aiming to compile a list of FAQs for all learning units, and your contributions will count towards your participation marks.

Improve this page! If you have questions or comments, please post them on the Quercus Discussion board with a subject line that includes the name of the unit.

References

Page ID: BIN-EXPR-Analysis

Author:
Boris Steipe ( <boris.steipe@utoronto.ca> )
Created:
2017-08-05
Last modified:
2022-09-14
Version:
1.1
Version History:
–  1.1 2020 Maintenance
–  1.0 First live version
–  0.1 First stub
Tagged with:
–  Unit
–  Live
–  Has lecture slides

 

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