BIN-GENOME-Genome Sequencing

Genome sequencing

 

Keywords:  Sequencing technologies, highly parallel, single-molecule and single-cell

 

 

 

Abstract

A basic introduction to "Next Generation Sequencing" concepts and technologies.

 

This unit ...

Prerequisites

You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources:

• Biomolecules: The molecules of life; nucleic acids and amino acids; the genetic code; protein folding; post-translational modifications and protein biochemistry; membrane proteins; biological function.

You need to complete the following units before beginning this one:

• BIN-Sequence (Sequence)

 

Objectives

This unit will ...

• ... introduce methods and concepts of "Next Generation Sequencing" and genome assembly.

 

Outcomes

After working through this unit you ...

• ... are familar with the basic methods and concepts of "Next Generation Sequencing" and genome assembly.

 

Deliverables

• Time management: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.
• Journal: Document your progress in your Course Journal. Some tasks may ask you to include specific items in your journal. Don't overlook these.
• Insights: If you find something particularly noteworthy about this unit, make a note in your insights! page.

 

Evaluation

Evaluation: NA

This unit is not evaluated for course marks.

 

Contents

Task:

• Read the introductory notes on genome-scale sequencing technologies.

 

Further reading, links and resources

A very informative and influential blog about the absolutely newest in the field is written by Lior Pachter, Berkley - "Bits of DNA". Check it out, and return frequently. (continuous)

For the newest published developments Nature subjects collection - NGS (continuous)

An EBI hands-on online course on NGS (2012)

 

Notes

 

Self-evaluation

 

 

---

 

If in doubt, ask! If anything about this learning unit is not clear to you, do not proceed blindly but ask for clarification. Post your question on the course mailing list: others are likely to have similar problems. Or send an email to your instructor.

---

 

About ...
 
Author:

Boris Steipe <boris.steipe@utoronto.ca>

Created:

2017-08-05

Modified:

2017-11-18

Version:

1.0

Version history:

• 1.0 First live 2017
• 0.1 First stub

---

This copyrighted material is licensed under a Creative Commons Attribution 4.0 International License. Follow the link to learn more.