BIN-SEQA-Collaboration

Sequence Analysis: Cooperation

 

Keywords:  Sequence analysis by cooperation (with R examples); Rosetta proteins; Interaction; Coexpression

 

 

Caution!

This unit is under development. There is some contents here but it is incomplete and/or may change significantly: links may lead to nowhere, the contents is likely going to be rearranged, and objectives, deliverables etc. may be incomplete or missing. Do not work with this material until it is updated to "live" status.

 

Abstract

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This unit ...

Prerequisites

You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources:

• Biomolecules: The molecules of life; nucleic acids and amino acids; the genetic code; protein folding; post-translational modifications and protein biochemistry; membrane proteins; biological function.

You need to complete the following units before beginning this one:

• BIN-PPI-Concepts
• BIN-SEQA-Concepts

 

Objectives

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Outcomes

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Deliverables

• Time management: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.
• Journal: Document your progress in your course journal.
• Insights: If you find something particularly noteworthy about this unit, make a note in your insights! page.

 

Evaluation

Evaluation: Integrated Unit

This unit should be submitted for evaluation for a maximum of 10 marks. Details TBD.

 

Contents

Co-Expression

Task:
CoExpressdb is a well curated database of pre-calculated co-expression profiles for model organisms. Expression values across a large number of published experiments on the same platform are compared via their coefficient of correlation. Highly correlated genes are either co-regulated, or one gene influences the expression level of the other.

• Navigate to CoExpressdb.
• Enter Mbp1 as a "gene alias" in the search field.
• Click on the link to the coexpressed gene list. Do any of the "known" target genes appear here? How do you interpret this result?

Unfortunately, the support for yeast genes is very limited. CoexDB is however an excellent resource to study higher eukaryotic, especially human genes. You might want to consider it for its additional capabilities for your "systems" term project. Refer to the YouTube tutorials for details.

 

 

Further reading, links and resources

Okamura et al. (2015) COXPRESdb in 2015: coexpression database for animal species by DNA-microarray and RNAseq-based expression data with multiple quality assessment systems. Nucleic Acids Res 43:D82-6. (pmid: 25392420)

[ PubMed ] [ DOI ] The COXPRESdb (http://coxpresdb.jp) provides gene coexpression relationships for animal species. Here, we report the updates of the database, mainly focusing on the following two points. For the first point, we added RNAseq-based gene coexpression data for three species (human, mouse and fly), and largely increased the number of microarray experiments to nine species. The increase of the number of expression data with multiple platforms could enhance the reliability of coexpression data. For the second point, we refined the data assessment procedures, for each coexpressed gene list and for the total performance of a platform. The assessment of coexpressed gene list now uses more reasonable P-values derived from platform-specific null distribution. These developments greatly reduced pseudo-predictions for directly associated genes, thus expanding the reliability of coexpression data to design new experiments and to discuss experimental results.

 

Notes

 

Self-evaluation

 

 

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If in doubt, ask! If anything about this learning unit is not clear to you, do not proceed blindly but ask for clarification. Post your question on the course mailing list: others are likely to have similar problems. Or send an email to your instructor.

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About ...
 
Author:

Boris Steipe <boris.steipe@utoronto.ca>

Created:

2017-08-05

Modified:

2017-08-05

Version:

0.1

Version history:

• 0.1 First stub

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