Difference between revisions of "BIN-GENOME-Genome Sequencing"

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<div id="ABC">
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<div style="padding:5px; border:1px solid #000000; background-color:#b3dbce; font-size:300%; font-weight:400; color: #000000; width:100%;">
 
Genome sequencing
 
Genome sequencing
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  </div>
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<div style="padding:5px; margin-top:20px; margin-bottom:10px; background-color:#b3dbce; font-size:30%; font-weight:200; color: #000000; ">
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(Sequencing technologies, highly parallel, single-molecule and single-cell)
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  {{Vspace}}
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</div>
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<div class="keywords">
 
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<b>Keywords:</b>&nbsp;
 
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Sequencing technologies, highly parallel, single-molecule and single-cell
 
 
</div>
 
</div>
  
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{{Smallvspace}}
  
  
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__TOC__
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<div style="padding:5px; border:1px solid #000000; background-color:#b3dbce33; font-size:85%;">
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<div style="font-size:118%;">
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{{Vspace}}
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<b>Abstract:</b><br />
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{{LIVE}}
 
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{{Vspace}}
 
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</div>
 
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<div id="ABC-unit-framework">
 
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== Abstract ==
 
 
<section begin=abstract />
 
<section begin=abstract />
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<!-- included from "./components/BIN-Genome-Sequencing.components.txt", section: "abstract" -->
 
 
A basic introduction to "Next Generation Sequencing" concepts and technologies.
 
A basic introduction to "Next Generation Sequencing" concepts and technologies.
 
<section end=abstract />
 
<section end=abstract />
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</div>
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{{Vspace}}
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<!-- ============================  -->
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<hr>
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<table>
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== This unit ... ==
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<tr>
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=== Prerequisites ===
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<td style="padding:10px;">
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<!-- included from "./components/BIN-Genome-Sequencing.components.txt", section: "prerequisites" -->
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<b>Objectives:</b><br />
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This unit will ...
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* ... introduce methods and concepts of "Next Generation Sequencing" and genome assembly.
 +
</td>
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<td style="padding:10px;">
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<b>Outcomes:</b><br />
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After working through this unit you ...
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* ... are familar with the basic methods and concepts of "Next Generation Sequencing" and genome assembly.
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</td>
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</tr>
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</table>
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<!-- ============================ -->
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<hr>
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<b>Deliverables:</b><br />
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<section begin=deliverables />
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<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-time_management" -->
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*<b>Time management</b>: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.
 +
<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-journal" -->
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*<b>Journal</b>: Document your progress in your [[FND-Journal|Course Journal]]. Some tasks may ask you to include specific items in your journal. Don't overlook these.
 +
<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-insights" -->
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*<b>Insights</b>: If you find something particularly noteworthy about this unit, make a note in your [[ABC-Insights|'''insights!''' page]].
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<section end=deliverables />
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<!-- ============================  -->
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<hr>
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<section begin=prerequisites />
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<b>Prerequisites:</b><br />
 
<!-- included from "./data/ABC-unit_components.txt", section: "notes-external_prerequisites" -->
 
<!-- included from "./data/ABC-unit_components.txt", section: "notes-external_prerequisites" -->
 
You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources:
 
You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources:
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*<b>Biomolecules</b>: The molecules of life; nucleic acids and amino acids; the genetic code; protein folding; post-translational modifications and protein biochemistry; membrane proteins; biological function.
 
*<b>Biomolecules</b>: The molecules of life; nucleic acids and amino acids; the genetic code; protein folding; post-translational modifications and protein biochemistry; membrane proteins; biological function.
 
<!-- included from "./data/ABC-unit_components.txt", section: "notes-prerequisites" -->
 
<!-- included from "./data/ABC-unit_components.txt", section: "notes-prerequisites" -->
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You need to complete the following units before beginning this one:
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This unit builds on material covered in the following prerequisite units:
 
*[[BIN-Sequence|BIN-Sequence (Sequence)]]
 
*[[BIN-Sequence|BIN-Sequence (Sequence)]]
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<section end=prerequisites />
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<!-- ============================  -->
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</div>
  
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{{Vspace}}
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{{Smallvspace}}
  
  
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=== Objectives ===
 
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<!-- included from "./components/BIN-Genome-Sequencing.components.txt", section: "objectives" -->
 
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This unit will ...
 
−
* ... introduce methods and concepts of "Next Generation Sequencing" and genome assembly.
 
  
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{{Vspace}}
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{{Smallvspace}}
  
  
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=== Outcomes ===
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__TOC__
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<!-- included from "./components/BIN-Genome-Sequencing.components.txt", section: "outcomes" -->
 
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After working through this unit you ...
 
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* ... are familar with the basic methods and concepts of "Next Generation Sequencing" and genome assembly.
 
−
 
 
−
{{Vspace}}
 
−
 
 
−
 
 
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=== Deliverables ===
 
−
<!-- included from "./components/BIN-Genome-Sequencing.components.txt", section: "deliverables" -->
 
−
<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-time_management" -->
 
−
*<b>Time management</b>: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.
 
−
<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-journal" -->
 
−
*<b>Journal</b>: Document your progress in your [[FND-Journal|Course Journal]]. Some tasks may ask you to include specific items in your journal. Don't overlook these.
 
−
<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-insights" -->
 
−
*<b>Insights</b>: If you find something particularly noteworthy about this unit, make a note in your [[ABC-Insights|'''insights!''' page]].
 
  
 
{{Vspace}}
 
{{Vspace}}
  
  
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</div>
 
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<div id="BIO">
 
 
== Contents ==
 
== Contents ==
 
<!-- included from "./components/BIN-Genome-Sequencing.components.txt", section: "contents" -->
 
<!-- included from "./components/BIN-Genome-Sequencing.components.txt", section: "contents" -->
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{{Vspace}}
 
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== Further reading, links and resources ==
 
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<!-- {{#pmid: 19957275}} -->
 
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<div class="reference-box">A very informative and influential blog about the absolutely newest in the field is written by [https://liorpachter.wordpress.com/ '''Lior Pachter''', Berkley - "Bits of DNA"]. Check it out, and return frequently. (continuous)</div>
 
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<div class="reference-box">For the newest ''published'' developments [https://www.nature.com/subjects/next-generation-sequencing '''Nature''' subjects collection - NGS] (continuous)</div>
 
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<div class="reference-box">[https://www.ebi.ac.uk/training/online/course/ebi-next-generation-sequencing-practical-course An EBI hands-on online course on NGS] (2012)</div>
 
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{{Vspace}}
 
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== Notes ==
 
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<!-- included from "./components/BIN-Genome-Sequencing.components.txt", section: "notes" -->
 
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<!-- included from "./data/ABC-unit_components.txt", section: "notes" -->
 
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<references />
 
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{{Vspace}}
 
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</div>
 
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<div id="ABC-unit-framework">
 
 
== Self-evaluation ==
 
== Self-evaluation ==
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<!-- included from "./components/BIN-Genome-Sequencing.components.txt", section: "self-evaluation" -->
 
 
<!--
 
<!--
 
=== Question 1===
 
=== Question 1===
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-->
 
-->
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== Notes ==
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<!-- included from "./components/BIN-Genome-Sequencing.components.txt", section: "notes" -->
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<!-- included from "./data/ABC-unit_components.txt", section: "notes" -->
 +
<references />
 +
== Further reading, links and resources ==
 +
<!-- {{#pmid: 19957275}} -->
 +
<div class="reference-box">A very informative and influential blog about the absolutely newest in the field is written by [https://liorpachter.wordpress.com/ '''Lior Pachter''', Berkley - "Bits of DNA"]. Check it out, and return frequently. (continuous)</div>
  
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{{Vspace}}
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<div class="reference-box">For the newest ''published'' developments [https://www.nature.com/subjects/next-generation-sequencing '''Nature''' subjects collection - NGS] (continuous)</div>
−
 
 
  
 +
<div class="reference-box">[https://www.ebi.ac.uk/training/online/course/ebi-next-generation-sequencing-practical-course An EBI hands-on online course on NGS] (2012)</div>
  
 
{{Vspace}}
 
{{Vspace}}

Revision as of 19:31, 26 January 2018

Genome sequencing

(Sequencing technologies, highly parallel, single-molecule and single-cell)


 


Abstract:

A basic introduction to "Next Generation Sequencing" concepts and technologies.

Objectives:
This unit will ...

  • ... introduce methods and concepts of "Next Generation Sequencing" and genome assembly.

Outcomes:
After working through this unit you ...

  • ... are familar with the basic methods and concepts of "Next Generation Sequencing" and genome assembly.

Deliverables:

  • Time management: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.
  • Journal: Document your progress in your Course Journal. Some tasks may ask you to include specific items in your journal. Don't overlook these.
  • Insights: If you find something particularly noteworthy about this unit, make a note in your insights! page.

Prerequisites:
You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources:

  • Biomolecules: The molecules of life; nucleic acids and amino acids; the genetic code; protein folding; post-translational modifications and protein biochemistry; membrane proteins; biological function.

This unit builds on material covered in the following prerequisite units:


 



 



 


Contents

Task:


Self-evaluation

Notes

Further reading, links and resources

A very informative and influential blog about the absolutely newest in the field is written by Lior Pachter, Berkley - "Bits of DNA". Check it out, and return frequently. (continuous)
For the newest published developments Nature subjects collection - NGS (continuous)
An EBI hands-on online course on NGS (2012)


 



 

If in doubt, ask! If anything about this learning unit is not clear to you, do not proceed blindly but ask for clarification. Post your question on the course mailing list: others are likely to have similar problems. Or send an email to your instructor.


 

About ...
 
Author:

Boris Steipe <boris.steipe@utoronto.ca>

Created:

2017-08-05

Modified:

2017-11-18

Version:

1.0

Version history:

  • 1.0 First live 2017
  • 0.1 First stub

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