Difference between revisions of "BIN-EXPR-Analysis"
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== Abstract == | == Abstract == | ||
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This unit introduces microarray and RNAseq methods to estimate mRNA expression levels in the cell. | This unit introduces microarray and RNAseq methods to estimate mRNA expression levels in the cell. | ||
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== This unit ... == | == This unit ... == | ||
=== Prerequisites === | === Prerequisites === | ||
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You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources: | You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources: | ||
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*<b>The Central Dogma</b>: Regulation of transcription and translation; protein biosynthesis and degradation; quality control. | *<b>The Central Dogma</b>: Regulation of transcription and translation; protein biosynthesis and degradation; quality control. | ||
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You need to complete the following units before beginning this one: | You need to complete the following units before beginning this one: | ||
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=== Objectives === | === Objectives === | ||
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This unit will ... | This unit will ... | ||
* ... introduce microarray and RNAseq methods to estimate mRNA expression levels in the cell. | * ... introduce microarray and RNAseq methods to estimate mRNA expression levels in the cell. | ||
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=== Outcomes === | === Outcomes === | ||
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After working through this unit you ... | After working through this unit you ... | ||
* ... are familar with key methods and terminology in the field. | * ... are familar with key methods and terminology in the field. | ||
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=== Deliverables === | === Deliverables === | ||
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*<b>Time management</b>: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit. | *<b>Time management</b>: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit. | ||
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*<b>Journal</b>: Document your progress in your [[FND-Journal|Course Journal]]. Some tasks may ask you to include specific items in your journal. Don't overlook these. | *<b>Journal</b>: Document your progress in your [[FND-Journal|Course Journal]]. Some tasks may ask you to include specific items in your journal. Don't overlook these. | ||
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*<b>Insights</b>: If you find something particularly noteworthy about this unit, make a note in your [[ABC-Insights|'''insights!''' page]]. | *<b>Insights</b>: If you find something particularly noteworthy about this unit, make a note in your [[ABC-Insights|'''insights!''' page]]. | ||
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<div id="BIO"> | <div id="BIO"> | ||
== Contents == | == Contents == | ||
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{{Task|1= | {{Task|1= | ||
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== Notes == | == Notes == | ||
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<references /> | <references /> | ||
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<div id="ABC-unit-framework"> | <div id="ABC-unit-framework"> | ||
== Self-evaluation == | == Self-evaluation == | ||
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=== Question 1=== | === Question 1=== | ||
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Revision as of 01:12, 6 January 2018
Expression Analysis
Keywords: Analysing expression measurements; Microarray: spots to levels; NGS: Reads to counts
Contents
Abstract
This unit introduces microarray and RNAseq methods to estimate mRNA expression levels in the cell.
This unit ...
Prerequisites
You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources:
- The Central Dogma: Regulation of transcription and translation; protein biosynthesis and degradation; quality control.
You need to complete the following units before beginning this one:
Objectives
This unit will ...
- ... introduce microarray and RNAseq methods to estimate mRNA expression levels in the cell.
Outcomes
After working through this unit you ...
- ... are familar with key methods and terminology in the field.
Deliverables
- Time management: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.
- Journal: Document your progress in your Course Journal. Some tasks may ask you to include specific items in your journal. Don't overlook these.
- Insights: If you find something particularly noteworthy about this unit, make a note in your insights! page.
Contents
Task:
- Read the introductory notes on measuring and analysing gene expression levels.
Further reading, links and resources
Notes
Self-evaluation
If in doubt, ask! If anything about this learning unit is not clear to you, do not proceed blindly but ask for clarification. Post your question on the course mailing list: others are likely to have similar problems. Or send an email to your instructor.
About ...
Author:
- Boris Steipe <boris.steipe@utoronto.ca>
Created:
- 2017-08-05
Modified:
- 2017-11-10
Version:
- 1.0
- 0.1
Version history:
- 1.0 First live version
- 0.1 First stub
This copyrighted material is licensed under a Creative Commons Attribution 4.0 International License. Follow the link to learn more.
