Difference between revisions of "BIN-EXPR-Analysis"

From "A B C"
Jump to navigation Jump to search
m
m
Line 1: Line 1:
 
<div id="ABC">
 
<div id="ABC">
<div style="padding:5px; border:1px solid #000000; background-color:#b3dbce; font-size:300%; font-weight:400; color: #000000; width:100%;">
+
<div style="padding:5px; border:1px solid #000000; background-color:#f4d7b7; font-size:300%; font-weight:400; color: #000000; width:100%;">
 
Expression Analysis
 
Expression Analysis
<div style="padding:5px; margin-top:20px; margin-bottom:10px; background-color:#b3dbce; font-size:30%; font-weight:200; color: #000000; ">
+
<div style="padding:5px; margin-top:20px; margin-bottom:10px; background-color:#f4d7b7; font-size:30%; font-weight:200; color: #000000; ">
 
(Analysing expression measurements; Microarray: spots to levels; NGS: Reads to counts)
 
(Analysing expression measurements; Microarray: spots to levels; NGS: Reads to counts)
 
</div>
 
</div>
Line 10: Line 10:
  
  
<div style="padding:5px; border:1px solid #000000; background-color:#b3dbce33; font-size:85%;">
+
<div style="padding:5px; border:1px solid #000000; background-color:#f4d7b733; font-size:85%;">
 
<div style="font-size:118%;">
 
<div style="font-size:118%;">
 
<b>Abstract:</b><br />
 
<b>Abstract:</b><br />
Line 38: Line 38:
 
<section begin=deliverables />
 
<section begin=deliverables />
 
<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-time_management" -->
 
<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-time_management" -->
*<b>Time management</b>: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.
+
<li><b>Time management</b>: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.</li>
 
<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-journal" -->
 
<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-journal" -->
*<b>Journal</b>: Document your progress in your [[FND-Journal|Course Journal]]. Some tasks may ask you to include specific items in your journal. Don't overlook these.
+
<li><b>Journal</b>: Document your progress in your [[FND-Journal|Course Journal]]. Some tasks may ask you to include specific items in your journal. Don't overlook these.</li>
 
<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-insights" -->
 
<!-- included from "./data/ABC-unit_components.txt", section: "deliverables-insights" -->
*<b>Insights</b>: If you find something particularly noteworthy about this unit, make a note in your [[ABC-Insights|'''insights!''' page]].
+
<li><b>Insights</b>: If you find something particularly noteworthy about this unit, make a note in your [[ABC-Insights|'''insights!''' page]].</li>
 
<section end=deliverables />
 
<section end=deliverables />
 
<!-- ============================  -->
 
<!-- ============================  -->
Line 49: Line 49:
 
<b>Prerequisites:</b><br />
 
<b>Prerequisites:</b><br />
 
<!-- included from "./data/ABC-unit_components.txt", section: "notes-external_prerequisites" -->
 
<!-- included from "./data/ABC-unit_components.txt", section: "notes-external_prerequisites" -->
You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources:
+
You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources:<br />
 
<!-- included from "./data/ABC-unit_prerequisites.txt", section: "central_dogma" -->
 
<!-- included from "./data/ABC-unit_prerequisites.txt", section: "central_dogma" -->
 
*<b>The Central Dogma</b>: Regulation of transcription and translation; protein biosynthesis and degradation; quality control.
 
*<b>The Central Dogma</b>: Regulation of transcription and translation; protein biosynthesis and degradation; quality control.
 
<!-- included from "./data/ABC-unit_components.txt", section: "notes-prerequisites" -->
 
<!-- included from "./data/ABC-unit_components.txt", section: "notes-prerequisites" -->
This unit builds on material covered in the following prerequisite units:
+
This unit builds on material covered in the following prerequisite units:<br />
 
<section end=prerequisites />
 
<section end=prerequisites />
 
<!-- ============================  -->
 
<!-- ============================  -->
Line 61: Line 61:
  
  
 +
{{REVISE}}
  
 
{{Smallvspace}}
 
{{Smallvspace}}

Revision as of 12:38, 16 September 2020

Expression Analysis

(Analysing expression measurements; Microarray: spots to levels; NGS: Reads to counts)


 


Abstract:

This unit introduces microarray and RNAseq methods to estimate mRNA expression levels in the cell.


Objectives:
This unit will ...

  • ... introduce microarray and RNAseq methods to estimate mRNA expression levels in the cell.

Outcomes:
After working through this unit you ...

  • ... are familar with key methods and terminology in the field.

Deliverables:

  • Time management: Before you begin, estimate how long it will take you to complete this unit. Then, record in your course journal: the number of hours you estimated, the number of hours you worked on the unit, and the amount of time that passed between start and completion of this unit.
  • Journal: Document your progress in your Course Journal. Some tasks may ask you to include specific items in your journal. Don't overlook these.
  • Insights: If you find something particularly noteworthy about this unit, make a note in your insights! page.

  • Prerequisites:
    You need the following preparation before beginning this unit. If you are not familiar with this material from courses you took previously, you need to prepare yourself from other information sources:

    • The Central Dogma: Regulation of transcription and translation; protein biosynthesis and degradation; quality control.

    This unit builds on material covered in the following prerequisite units:


     


    This page is tagged for revision; expect changes and proceed with caution.


     



     


    Contents

    Task:

    Self-evaluation

    Notes

    Further reading, links and resources


     




     

    If in doubt, ask! If anything about this learning unit is not clear to you, do not proceed blindly but ask for clarification. Post your question on the course mailing list: others are likely to have similar problems. Or send an email to your instructor.



     

    About ...
     
    Author:

    Boris Steipe <boris.steipe@utoronto.ca>

    Created:

    2017-08-05

    Modified:

    2017-11-10

    Version:

    1.0
    0.1

    Version history:

    • 1.0 First live version
    • 0.1 First stub

    CreativeCommonsBy.png This copyrighted material is licensed under a Creative Commons Attribution 4.0 International License. Follow the link to learn more.